Question 1
The photograph shows an animal called a margay.

Four processes that occur in the margay are listed:
- excretion
- growth
- digestion
- respiration
How many of the processes that occur in the margay are characteristics of all living organisms?
(B) $2$
(C) $3$
(D) $4$
▶️ Answer/Explanation
✅ Answer: (C)
Question 2
Part of the base sequence of DNA from four species was determined.
species $2$ T G A G C A A T
species $3$ T G A G C A A G
species $4$ T G A G G A T T
Which two species are the least closely related?
▶️ Answer/Explanation
To determine which species are the least closely related, we must count the number of differences in their DNA base sequences. Species with the highest number of differences share a more distant common ancestor. Comparing species $1$ (T C A G G A T T) and species $3$ (T G A G C A A G), we find differences at the $2^{nd}$, $5^{th}$, $6^{th}$, $7^{th}$, and $8^{th}$ positions, totaling $5$ differences.
In contrast, other pairs have fewer differences: species $1$ and $2$ have $4$ differences, while species $2$ and $3$ differ by only $1$ base. Because the DNA sequence of species $1$ and $3$ is the most divergent among the choices provided, they are the least closely related.
✅ Answer: (B)
Question 3
A single-celled organism has chloroplasts, a cell wall and a nucleus.
Which kingdom is this organism placed in?
(B) plant
(C) prokaryote
(D) protoctist
▶️ Answer/Explanation
✅ Answer: (D)
Question 4
What is a leaf?
(B) an organ
(C) an organ system
(D) a tissue
▶️ Answer/Explanation
In the hierarchy of biological organization, an organ is defined as a collection of different tissues that work together to perform specific functions. A leaf fits this description perfectly because it is composed of several specialized tissues, including the epidermis, mesophyll (photosynthetic tissue), and vascular tissues (xylem and phloem). These tissues collaborate to carry out essential processes like photosynthesis and transpiration. While a single cell is the basic unit of life and a tissue is a group of similar cells, the leaf represents a higher level of complexity. Therefore, it is classified as an organ within the plant’s shoot system.
✅ Answer: (B)
Question 5
A student measured the length of a structure using a microscope.
The magnification used was $\times 250$.
The image size of the structure was $5.00 \text{ mm}$.
What was the actual length of the structure?
(B) $1.25 \times 10^3 \text{ \mu m}$
(C) $2.00 \times 10^1 \text{ \mu m}$
(D) $2.00 \times 10^{-5} \text{ \mu m}$
▶️ Answer/Explanation
✅ Answer: (C)
Question 6
Which row shows the conditions when diffusion of oxygen into the blood is fastest?

▶️ Answer/Explanation
✅ Answer: (A)
Question 7
Uncooked potato cylinders of identical size were placed in four different liquids for two hours.
The different liquids are listed.
2 a sugar solution that was less concentrated than the contents of the potato cells
3 a sugar solution that was more concentrated than the contents of the potato cells
4 a sugar solution that was the same concentration as the contents of the potato cells
In which liquids will the potato cylinders increase in length?
▶️ Answer/Explanation
This question relates to osmosis, the movement of water from a region of higher water potential to a region of lower water potential across a partially permeable membrane. To increase in length, the potato cells must take in water and become turgid. This occurs when the surrounding liquid is hypotonic (has a higher water potential) relative to the cell sap.
In Liquid 1 (pure water) and Liquid 2 (a dilute sugar solution), the water potential outside the potato is higher than inside. Consequently, water moves into the potato cylinders, causing them to expand. In contrast, Liquid 3 is hypertonic (causing water loss and shrinkage), and Liquid 4 is isotonic (resulting in no net change in length).
✅ Answer: (A)
Question 8
What is the correct definition of enzymes?
(B) carbohydrates that act as substrates
(C) proteins that act as biological catalysts
(D) proteins that act as substrates
▶️ Answer/Explanation
Enzymes are specialized proteins synthesized by living cells to speed up metabolic reactions. They function as biological catalysts, meaning they lower the activation energy required for a reaction to occur without being consumed in the process. While carbohydrates serve as energy sources or structural components, they do not possess catalytic properties. Similarly, a substrate is the specific molecule that an enzyme acts upon, rather than the enzyme itself. Therefore, the most accurate definition identifies them as protein-based catalysts.
✅ Answer: (C)
Question 9
A student investigated how the rate of photosynthesis is affected by carbon dioxide concentration, temperature and light intensity.
The graphs show the results.

What is a correct statement for these results?
(B) No enzymes are denatured at $3$.
(C) Light intensity is a limiting factor at $4$.
(D) Temperature is a limiting factor at $2$.
▶️ Answer/Explanation
✅ Answer: (A)
Question 10
The diagram shows a cross-section of part of a leaf.

What is the cell labelled $X$?
(B) guard cell
(C) palisade mesophyll
(D) spongy mesophyll
▶️ Answer/Explanation
✅ Answer: (C) palisade mesophyll
Question 11
In which parts of the digestive system does physical digestion occur?

(B) $1$ and $2$ only
(C) $2$ and $3$ only
(D) $4$ only
▶️ Answer/Explanation
✅ Answer: (B)
Question 12
The table shows the substrates and end products in four enzyme-controlled chemical reactions.
Which reaction was controlled by lipase?

▶️ Answer/Explanation
✅ Answer: (B)
Question 13
What is the function of microvilli in the small intestine?
(B) increasing surface area for absorption
(C) movement of food particles
(D) movement of mucus
▶️ Answer/Explanation
✅ Answer: (B)
Question 14
Which row shows the features of xylem vessels?

▶️ Answer/Explanation
Xylem vessels are specialized tissues designed for the efficient transport of water and mineral ions. To withstand the high pressure of the transpiration stream, their walls are thickened with a tough, waterproof substance called lignin. Crucially, mature xylem vessels are dead cells; they lose their protoplasm, including organelles like mitochondria, to form hollow, continuous tubes. This lack of internal structure ensures there is no resistance to the flow of water. Therefore, they have thick walls and no mitochondria.
✅ Answer: (B)
Question 15
A student wanted to investigate the effect of humidity on transpiration. She set up two sets of apparatus with identical-sized plants of the same species.

The masses of the water in both tubes were measured at the start of the investigation and again after five days. The table shows the results of the investigation.

Which statement describes and explains these results?
(B) Transpiration in tube $Y$ was higher than in tube $X$ as the plastic bag increased the humidity.
(C) Transpiration in tube $Y$ was lower than in tube $X$ as the plastic bag decreased the humidity.
(D) Transpiration in tube $Y$ was lower than in tube $X$ as the plastic bag increased the humidity.
▶️ Answer/Explanation
✅ Answer: (D)
Question 16
What is an advantage of a double circulatory system?
(B) It can keep blood pressure high in the lungs.
(C) Less carbon dioxide is removed from the body cells.
(D) Oxygen is supplied to body cells by blood at high pressure.
▶️ Answer/Explanation
In a double circulatory system, blood passes through the heart twice for every one complete circuit of the body. This separation allows the heart to pump blood to the lungs at a lower pressure to protect delicate tissues, while pumping blood to the rest of the body at a much higher pressure.
By re-pressurizing the blood after it returns from the lungs, the system ensures that oxygenated blood reaches systemic tissues rapidly and efficiently. This supports a higher metabolic rate, which is essential for maintaining body temperature and high levels of activity. Options A and C describe disadvantages or inefficiencies, while B is incorrect because lung capillaries require lower pressure to prevent fluid buildup.
✅ Answer: (D)
Question 17
Which structure in the heart separates oxygenated and deoxygenated blood?
(B) muscle wall of left atrium
(C) semilunar valve
(D) septum
▶️ Answer/Explanation
✅ Answer: (D)
Question 18
Which row shows the features of a vein?

▶️ Answer/Explanation
Veins are blood vessels that carry blood toward the heart under low pressure. Because the pressure is significantly lower than in arteries, veins have relatively thin walls. To facilitate blood flow with minimal resistance, they possess a large lumen (internal diameter). Additionally, because blood in veins often travels against gravity, they contain valves to prevent the backflow of blood, ensuring it moves in one direction. These three characteristics—thin walls, large lumen, and the presence of valves—are correctly identified in row D.
✅ Answer: (D)
Question 19
Three statements about immunity are listed.
1 The transfer of antibodies from mother to baby in breast milk is an example of passive immunity.
2 Passive immunity results in long-term immunity because of the production of memory cells.
3 Active immunity is gained after vaccination with antigens.
Which statements are correct?
(B) $1$ and $2$ only
(C) $1$ and $3$ only
(D) $2$ and $3$ only
▶️ Answer/Explanation
Statement $1$ is correct because passive immunity involves receiving ready-made antibodies, such as those passed through breast milk. Statement $2$ is incorrect because passive immunity is short-term; since the body does not produce the antibodies itself, no memory cells are created. Statement $3$ is correct as active immunity occurs when the immune system is triggered by antigens (via infection or vaccination) to produce its own antibodies and memory cells. Therefore, only statements $1$ and $3$ are accurate.
✅ Answer: (C)
Question 20
The table shows the breathing rate and the average total volume of air breathed by a student when exercising for $20$ minutes.

Which conclusion can be made from the data in the table about the effect of exercise on breathing?
(B) Between $0$ minutes and $10$ minutes, the depth of breathing increases.
(C) Between $10$ minutes and $20$ minutes, there is no change to the rate of breathing.
(D) Between $15$ minutes and $20$ minutes, the depth of breathing decreases.
▶️ Answer/Explanation
✅ Answer: (B)
Question 21
What is the chemical formula of glucose?
(B) $C_{6}HO_{6}$
(C) $C_{6}H_{12}O_{6}$
(D) $C_{12}H_{12}O_{6}$
▶️ Answer/Explanation
Glucose is a simple sugar and a vital monosaccharide used as a primary energy source in biological organisms. Its molecular structure consists of six carbon atoms, twelve hydrogen atoms, and six oxygen atoms. This ratio follows the general formula for carbohydrates, which is $C_{n}(H_{2}O)_{n}$.
In the case of glucose, where $n=6$, the formula becomes $C_{6}H_{12}O_{6}$. Option (A) lacks sufficient hydrogen, option (B) is missing most hydrogen atoms, and option (D) incorrectly doubles the carbon count. Therefore, option (C) is the only scientifically accurate representation of a glucose molecule.
✅ Answer: (C)
Question 22
After a race, athletes experience oxygen debt.
The diagram shows how the oxygen debt is removed.

What happens at $X$?
(B) aerobic respiration of lactic acid
(C) anaerobic respiration of glucose
(D) anaerobic respiration of lactic acid
▶️ Answer/Explanation
✅ Answer: (B)
Question 23
Which organ produces urea?
(B) kidney
(C) liver
(D) pancreas
▶️ Answer/Explanation
✅ Answer: (C)
Question 24
The diagram shows a vertical section through part of a human eye. A fly is coming nearer to the eye. The eye begins to focus the image of the fly on the retina.

How do the labelled parts of the diagram change?

▶️ Answer/Explanation
✅ Answer: (C)
Question 25
Which organ produces glucagon?
(B) liver
(C) ovary
(D) pancreas
▶️ Answer/Explanation
✅ Answer: (D)
Question 26
A young seedling is growing underground.
The shoot grows towards the surface of the ground.
The root grows away from the surface of the ground.
Which row describes the responses involved?

▶️ Answer/Explanation
✅ Answer: (A)
Question 27
Clostridium difficile is a species of bacterium that is resistant to many antibiotics.
From $2002$ to $2006$, the number of people in one country infected with Clostridium difficile increased from $1300$ to $6200$ people.
What was the percentage increase in the number of people infected with Clostridium difficile from $2002$ to $2006$ to the nearest whole number?
(B) $27\%$
(C) $79\%$
(D) $377\%$
▶️ Answer/Explanation
First, calculate the actual increase in the number of people: $6200 – 1300 = 4900$.
Next, divide this increase by the original population from $2002$: $\frac{4900}{1300} \approx 3.7692$.
Multiplying by $100$ gives $376.92\%$.
When rounded to the nearest whole number, we get $377\%$. This represents a significant growth in infections over the four-year period.
✅ Answer: (D)
Question 28
What are the characteristics of asexual reproduction?

▶️ Answer/Explanation
Asexual reproduction involves a single parent and does not require the fusion of gametes (fertilization), so the answer for the first column is no. Since there is no mixing of genetic material from two different parents, the offspring are genetically identical to the parent and to each other (clones). Therefore, there is no genetic variety in the offspring, making the answer for the second column no. This biological process typically relies on mitosis to ensure the DNA is replicated exactly. Only sexual reproduction involves the fusion of nuclei and results in genetic variation.
✅ Answer: (D)
Question 29
Which description of cross-pollination is correct?
(B) the transfer of pollen grains from the anther to the stigma on the same plant
(C) the transfer of pollen grains from the stigma of one plant to the anther on a different plant
(D) the transfer of pollen grains from the stigma to the anther on the same plant
▶️ Answer/Explanation
✅ Answer: (A)
Question 30
Which row correctly links the named process with its description?

▶️ Answer/Explanation
Menstruation is the phase of the menstrual cycle where the thickened uterine lining (endometrium) breaks down and is discharged from the body. Row B accurately describes this process. In contrast, the “release of an egg” defines ovulation, not menstruation. Furthermore, the “fusion of nuclei” refers to fertilization, and while ovulation occurs after puberty begins, it is a specific monthly event rather than the definition of puberty itself.
✅ Answer: (B)
Question 31
The diagram shows the inheritance of a genetic condition in one family.

What can be concluded about this genetic condition?
(B) It is caused by a dominant allele.
(C) It is caused by recessive alleles.
(D) The alleles for the condition must be sex-linked.
▶️ Answer/Explanation
✅ Answer: (B)
Question 32
What is a way in which new alleles are formed?
(B) mutation
(C) natural selection
(D) sexual reproduction
▶️ Answer/Explanation
New alleles are created exclusively through mutation, which is a permanent change in the $DNA$ sequence of a gene. While sexual reproduction (D) increases genetic variation by shuffling existing alleles through recombination and independent assortment, it does not create “new” genetic information. Similarly, natural selection (C) and artificial selection (A) only act upon the variations that are already present in a population, favoring certain traits over others. Therefore, mutation is the primary source of all new genetic material and the ultimate driver of evolutionary novelty.
✅ Answer: (B)
Question 33
How does artificial selection differ from natural selection?
(B) Artificial selection is based on genetic variation.
(C) Artificial selection does not involve competition for resources.
(D) Artificial selection occurs over many generations.
▶️ Answer/Explanation
Both natural and artificial selection rely on genetic variation and change the characteristics of populations over generations. However, natural selection is driven by environmental pressures where individuals must compete for limited resources to survive and reproduce. In contrast, artificial selection (selective breeding) is controlled by humans who choose specific individuals for breeding based on desired traits. Because humans provide the necessary food and shelter for these chosen organisms, the survival-of-the-fittest competition seen in the wild is largely removed. Therefore, the absence of resource competition is a key distinguishing factor.
✅ Answer: (C)
Question 34
Which diagram of a xerophyte is labelled correctly?

▶️ Answer/Explanation
✅ Answer: (A)
Question 35
Which row describes the energy flow into and through a food chain that starts with a plant?

▶️ Answer/Explanation
Energy enters most food chains as light energy from the Sun, which plants (producers) capture during photosynthesis to create glucose. This light energy is converted and stored as chemical energy within the organic molecules of the plant. When a consumer eats the plant, or another animal, this stored chemical energy is what gets transferred along the chain. While energy is lost as heat to the environment at each level, the actual transfer of useful energy between organisms occurs in chemical form.
✅ Answer: (C)
Question 36
In a food chain, zebras are large mammals that eat grass. The zebras have many insects living on them which suck their blood. Birds eat the insects.
The diagrams show some biological pyramids.

Which row identifies the types of pyramids for this food chain?

▶️ Answer/Explanation
✅ Answer: (C)
Question 37
The diagram shows part of the nitrogen cycle.

What is the process labelled $X$ in the diagram?
(B) denitrification
(C) nitrification
(D) nitrogen fixation
▶️ Answer/Explanation
✅ Answer: (C)
Question 38
The graph shows the number of water fleas in a newly-created pond.

Which event could be responsible for the shape of the graph at $Y$?
(B) an increase in the birth rate of the water fleas
(C) an increase in the food supply for the water fleas
(D) the addition of predators which feed on water fleas
▶️ Answer/Explanation
✅ Answer: (D)
Question 39
When managing fish stocks as a sustainable resource, what is a reason for harvesting only the larger fish of a particular species?
(B) Larger fish sell at a higher price.
(C) Smaller fish are eaten by predators.
(D) Smaller fish are too young to breed.
▶️ Answer/Explanation
✅ Answer: (D)
Question 40
Which features make bacteria useful in biotechnology?
$2$ They do not contain plasmids.
$3$ They make complex molecules.
$4$ They reproduce rapidly.
▶️ Answer/Explanation
Bacteria are ideal for biotechnology because they can be engineered to synthesize complex molecules, such as human insulin, which would be difficult to produce chemically. Their ability to reproduce rapidly through binary fission allows for the quick mass production of these desired substances in large fermenters.
Conversely, statements $1$ and $2$ are incorrect: bacteria are generally susceptible to antibiotics (unless specifically engineered for resistance markers), and their plasmids are actually essential tools in biotechnology for transferring foreign DNA. Therefore, only points $3$ and $4$ are correct.
✅ Answer: (D)
