Question 1
The photographs show two different rats

Which statement about the rats is correct?
(B) The rats belong to the same species.
(C) The rats can breed together to produce fertile offspring.
(D) The rats do not share any of the same features.
▶️ Answer/Explanation
✅ Answer: (A)
Question 2
The diagrams show cells of organisms from three different kingdoms.

Which row shows the kingdoms for the organism cells labelled $1$, $2$ and $3$?

▶️ Answer/Explanation
Cell $1$ represents a fungus (specifically yeast), identifiable by its cell wall, nucleus, and large vacuole. Cell $2$ is a prokaryote (bacterium), characterized by the lack of a membrane-bound nucleus, a single loop of DNA, and the presence of flagella. Cell $3$ is a protoctist (like an Amoeba), as it is a eukaryotic unicellular organism containing a distinct nucleus and mitochondria but lacking a rigid cell wall. Matching these identifications to the table, only row B correctly categorizes all three organisms based on their cellular structures.
✅ Answer: (B)
Question 3
The diagram shows a plant cell.
A biologist wants to find out the number of chromosomes it contains.
Which labelled part should be examined more closely?

▶️ Answer/Explanation
✅ Answer: (A)
Question 4
Which term describes each structure?

▶️ Answer/Explanation
Biological organization follows a hierarchy: cells group to form tissues, and tissues group to form organs. A neurone is a single nerve cell, which is the basic functional unit of the nervous system. The brain and the liver are both complex structures made of multiple types of tissues working together to perform specific physiological functions, classifying them both as organs. Row A correctly identifies both the brain and liver as organs and the neurone as a cell.
✅ Answer: (A)
Question 5
The diagram shows the order of bases along part of one strand of DNA.
What is the order of bases on the complementary part of the other strand?
(B) C—A—G—G—C—T—C—G
(C) T—A—G—A—T—T—C—A
(D) T—G—A—A—T—C—T—A
▶️ Answer/Explanation
✅ Answer: (D)
Question 6
Which substance is an enzyme?
(B) mRNA
(C) pepsin
(D) saliva
▶️ Answer/Explanation
✅ Answer: (C)
Question 7
Which function is performed in the duodenum?
(B) digestion
(C) egestion
(D) ingestion
▶️ Answer/Explanation
✅ Answer: (B)
Question 8
The graph shows the changes in $pH$ in the oesophagus, stomach and duodenum.

Which substance causes the change in $pH$ at the point labelled $X$?
(B) gastric juice
(C) glucagon
(D) trypsin
▶️ Answer/Explanation
✅ Answer: (A)
Question 9
Which row names a small molecule and where that molecule is absorbed in the digestive system?

▶️ Answer/Explanation
To solve this, we must identify where specific molecules are absorbed. Amino acids and glucose are primarily absorbed in the small intestine, making rows A and B incorrect. Maltose is a disaccharide that must be broken down into glucose before absorption, so row C is incorrect. Water is unique because it is absorbed via osmosis in both the small intestine (where the majority of absorption occurs) and the colon (where remaining water is reclaimed). Therefore, row D correctly identifies a small molecule and its absorption sites.
✅ Answer: (D)
Question 10
A student cut one stalk of celery from a celery plant.
They put the celery stalk into a beaker of blue dye.
The celery stalk was removed from the blue dye after two hours and was cut into sections.

What is shown by the blue circles in the cut sections of celery stalk?
(B) position of the palisade cells
(C) position of the phloem cells
(D) position of the xylem vessels
▶️ Answer/Explanation
✅ Answer: (D)
Question 11
Which process moves water from the surfaces of the mesophyll cells into the air spaces during transpiration?
(B) circulation
(C) evaporation
(D) osmosis
▶️ Answer/Explanation
✅ Answer: (C)
Question 12
The mass of water lost from a plant was investigated. The leaves of the plant were covered with a type of grease that acts as a waterproof barrier. The environmental conditions remained the same throughout the experiment. The table shows the results of the investigation.
| treatment | mass lost in seven days / $g$ |
|---|---|
| no grease applied | $12.0$ |
| grease applied only to the upper surface of every leaf | $8.7$ |
| grease applied to both surfaces of every leaf | $0.0$ |
What is the mean daily rate of water loss through the upper surface of the leaves?
(B) $1.24 \text{ g / day}$
(C) $1.71 \text{ g / day}$
(D) $3.30 \text{ g / day}$
▶️ Answer/Explanation
✅ Answer: (A)
Question 13
What is translocation?
(B) the movement of starch and water from source to sink
(C) the movement of sucrose and amino acids from source to sink
(D) the movement of sucrose and mineral ions from sink to source
▶️ Answer/Explanation
Translocation is the process by which organic molecules, primarily sucrose and amino acids, are transported through the phloem of a plant. This movement occurs from “sources,” such as photosynthesizing leaves, to “sinks,” such as growing roots or developing fruits. Unlike transpiration, which only moves water and minerals upward in the xylem, translocation can move substances in multiple directions depending on where the plant needs energy. Starch is not moved directly because it is insoluble; it must be converted to sucrose first. Therefore, the transport of sucrose and amino acids from source to sink defines the process.
✅ Answer: (C)
Question 14
Which row names the blood vessel containing the highest blood urea concentration and the blood vessel containing the lowest blood urea concentration?

▶️ Answer/Explanation
✅ Answer: (B)
Question 15
The diagram shows the human digestive system.
Where do cholera bacteria cause the secretion of chloride ions?

▶️ Answer/Explanation
✅ Answer: (D)
Question 16
Which row describes what happens when breathing in?

▶️ Answer/Explanation
✅ Correct Answer: (A)
Question 17
A person exercised vigorously for $1$ minute and then rested for $1$ hour.
Which statements about lactic acid are correct?
$2$ The blood transports lactic acid to the liver.
$3$ Lactic acid is broken down by anaerobic respiration.
(B) $1$ and $2$ only
(C) $2$ and $3$ only
(D) $2$ only
▶️ Answer/Explanation
During vigorous exercise, muscle cells perform anaerobic respiration when oxygen supply is insufficient, producing lactic acid which then diffuses into the bloodstream (Statement $1$). The blood carries this lactic acid to the liver to be processed (Statement $2$). However, Statement $3$ is incorrect because lactic acid is broken down or converted back into glucose through aerobic metabolism (repaying the “oxygen debt”), not anaerobic. Since only statements $1$ and $2$ are scientifically accurate, the correct option is B.
✅ Answer: (B)
Question 18
The diagram shows the parts of the nervous system involved in a reflex arc.
Which letter identifies a synapse?

▶️ Answer/Explanation
✅ Answer: (D)
Question 19
A person looks at a distant object in a dark area. The person then moves to a bright area and looks at a near object.
Which statement describes some of the changes that take place in the person’s eyes?
(A) The circular muscles of the iris contract, the pupil constricts, the ciliary muscles relax and the lens gets thicker.
(B) The radial muscles of the iris relax, the circular muscles of the iris contract, the pupil constricts and the lens gets thinner.
(C) The radial muscles of the iris relax, the pupil constricts, the ciliary muscles contract and the lens gets thicker.
(D) The radial muscles of the iris relax, the pupil dilates, the ciliary muscles relax and the lens gets thicker.
▶️ Answer/Explanation
This question involves two physiological mechanisms: the pupillary light reflex and accommodation.
1. Dark to Bright Area: To restrict the amount of light entering the eye, the pupil must constrict. This is achieved when the circular muscles of the iris contract and the radial muscles relax.
2. Distant to Near Object: To focus on a near object, the lens must become more convex (thicker) to increase refraction. This happens when the ciliary muscles contract, which releases tension on the suspensory ligaments, allowing the lens to bulge and get thicker.
Option C correctly identifies both the relaxation of radial muscles (for constriction in bright light) and the contraction of ciliary muscles leading to a thicker lens (for near vision).
✅ Answer: (C)
Question 20
Which effect does adrenaline have on blood glucose concentration and pulse rate?

▶️ Answer/Explanation
Adrenaline is known as the “fight or flight” hormone, secreted by the adrenal glands during stressful or dangerous situations. It acts to increase the supply of oxygen and glucose to the brain and muscles. By stimulating the liver to convert glycogen into glucose, it increases the blood glucose concentration. Simultaneously, it stimulates the pacemaker of the heart, which increases the pulse rate to pump blood faster throughout the body. Therefore, both parameters show an upward trend to prepare the body for immediate physical action.
✅ Answer: (D)
Question 21
The diagram shows an experiment using two wheat shoot tips, X and Y.

Which statement is supported by the evidence provided by this experiment?
(B) Auxin is made in the shoot tip.
(C) Auxin is unequally distributed in response to light.
(D) Auxin inhibits cell elongation.
▶️ Answer/Explanation
✅ Answer: (B)
Question 22
Which action would help to limit the development of antibiotic resistance in bacteria?
(B) Use antibiotics only to treat bacterial infections.
(C) Use antibiotics to treat all types of infections.
(D) Use antibiotics to treat only viral infections.
▶️ Answer/Explanation
✅ Answer: (B)
Question 23
What is an example of asexual reproduction?
(B) a mammal giving birth to $4$ offspring
(C) a female bird laying $6$ eggs which will hatch into the same species
(D) a tomato plant producing fruits which contain many seeds
▶️ Answer/Explanation
✅ Answer: (A)
Question 24
The diagram shows parts of two different plants, $1$ and $2$, of the same species. The transfer of pollen between the flowers of these two plants is shown by the arrows labelled $P$ and $Q$.

Which arrows show cross-pollination, and which transfer of pollen would produce the most variation in the offspring?

▶️ Answer/Explanation
✅ Answer: (A)
Question 25
Which substance is a hormone that is involved in the development and regulation of male secondary sexual characteristics?
(B) glucagon
(C) lactase
(D) testosterone
▶️ Answer/Explanation
Testosterone is the primary male sex hormone produced mainly in the testes. It is responsible for the development of male secondary sexual characteristics such as increased muscle mass, deepening of the voice, and the growth of body hair during puberty.
In contrast, insulin and glucagon are hormones produced by the pancreas to regulate blood glucose levels. Lactase is not a hormone at all; it is an enzyme used to break down lactose in the digestive system. Therefore, testosterone is the only option that fits the biological role described.
✅ Answer: (D)
Question 26
Which statement describes the net direction of movement of a substance across the placenta?
(B) Glucose moves from the fetus’s blood to the mother’s blood.
(C) Glycogen moves from the mother’s blood to the fetus’s blood.
(D) Urea moves from the fetus’s blood to the mother’s blood.
▶️ Answer/Explanation
✅ Answer: (D)
Question 27
The diagram shows the human female reproductive system.
Which label identifies the uterus?

▶️ Answer/Explanation
✅ Answer: (C)
Question 28
Which methods can transmit HIV?
2 sharing a needle with a person infected with HIV
3 using a plate that a person infected with HIV has used
(B) $1$ only
(C) $2$ and $3$ only
(D) $2$ only
▶️ Answer/Explanation
✅ Answer: (D)
Question 29
Which chromosomes can be found in a single sperm?
(B) $X$ and $Y$
(C) $X$ or $X$
(D) $X$ or $Y$
▶️ Answer/Explanation
In humans, somatic cells are diploid and contain two sex chromosomes ($XY$ in males). During the process of meiosis, these pairs separate to form haploid gametes. Since a sperm cell is a gamete, it contains only half the genetic material of a normal cell, meaning it carries only one sex chromosome.
Approximately $50\%$ of sperm cells will carry an $X$ chromosome, and the other $50\%$ will carry a $Y$ chromosome. Therefore, any single sperm will contain either an $X$ or a $Y$ chromosome, but never both under normal biological conditions. Options suggesting “and” are incorrect because they imply the sperm is diploid for sex chromosomes.
✅ Answer: (D)
Question 30
Why are lymphocytes the only cells that produce antibody proteins?
(B) Lymphocytes have twice as many genes as other cells.
(C) Other cells do not have the genes for antibodies.
(D) The genes for antibodies are only expressed in lymphocytes.
▶️ Answer/Explanation
✅ Answer: (D)
Question 31
During protein synthesis, what is the function of the ribosome?
(B) It carries a copy of a gene to the cytoplasm.
(C) It contains the code for the synthesis of a protein.
(D) It determines the order of bases in the protein.
▶️ Answer/Explanation
✅ Answer: (A)
Question 32
The photograph shows a speckled chicken. Speckled chickens have white feathers and black feathers.

When a chicken with only white feathers and a chicken with only black feathers are crossed, all of the offspring are speckled.
What would be the expected phenotypic ratio if a speckled chicken was crossed with a chicken with only white feathers?
(B) all offspring are speckled
(C) $1$ white feathers only : $1$ speckled
(D) $3$ white feathers only : $1$ speckled
▶️ Answer/Explanation
This is an example of codominance, where both the black ($B$) and white ($W$) alleles are expressed equally in the phenotype. The speckled chicken has the genotype $BW$, while the white chicken is $WW$. When these are crossed ($BW \times WW$), the resulting Punnett square yields two $BW$ (speckled) and two $WW$ (white) offspring. This results in a phenotypic ratio of $1$ white : $1$ speckled.
✅ Answer: (C)
Question 33
What are stem cells?
(B) specialised cells that divide by mitosis to produce daughter cells
(C) unspecialised cells that divide by meiosis to produce daughter cells
(D) unspecialised cells that divide by mitosis to produce daughter cells
▶️ Answer/Explanation
Stem cells are defined by their unique ability to remain unspecialised, meaning they do not yet have a specific structure or function like a muscle or nerve cell. They maintain their population and growth through mitosis, a process of cell division that results in two genetically identical daughter cells. Meiosis is excluded here because it is strictly for the production of gametes (sex cells) and reduces the chromosome number by half. Consequently, stem cells are the “blank slates” of the body that use mitotic division to either self-renew or differentiate into various specialised cell types.
✅ Answer: (D)
Question 34
What are features of hydrophytes which have leaves that float?
(B) many stomata on upper surface of leaves
(C) hairy leaves
(D) small air spaces in the spongy mesophyll
▶️ Answer/Explanation
Hydrophytes with floating leaves, like water lilies, have specialized adaptations for an aquatic environment. Unlike land plants that have stomata primarily on the underside to prevent water loss, floating leaves have many stomata on the upper surface to facilitate gas exchange directly with the atmosphere. They also possess large air spaces (aerenchyma) in the mesophyll rather than small ones to provide buoyancy. A long root system is unnecessary because water is abundant, and hairy leaves are typically an adaptation for xerophytes to trap moisture, which is not a concern here.
✅ Answer: (B)
Question 35
Which unit could be used for the measurements needed to construct a pyramid of energy?
(B) $\text{kJ}$ per $\text{kg}$ per year
(C) $\text{kJ}$ per $\text{m}^2$ per year
(D) $\text{kJ}$ per $\text{kg}$
▶️ Answer/Explanation
A pyramid of energy shows the flow of energy through trophic levels in an ecosystem. Unlike pyramids of numbers or biomass, which represent a “snapshot” in time, energy pyramids represent the rate of energy production. This requires three components: energy ($\text{kJ}$), area ($\text{m}^2$), and time (year). By measuring energy per unit area per unit time, we can accurately compare the efficiency of energy transfer between different levels, accounting for seasonal variations. Therefore, the standard unit is $\text{kJ}\text{ m}^{-2}\text{ yr}^{-1}$.
✅ Answer: (C)
Question 36
Which process needs microorganisms to break down protein to produce ammonium ions?
(B) denitrification
(C) nitrification
(D) nitrogen fixation
▶️ Answer/Explanation
✅ Answer: (A)
Question 37
The graph shows part of a growth curve for a bacterial population.

What is not shown in the graph?
(B) the exponential phase
(C) the lag phase
(D) the stationary phase
▶️ Answer/Explanation
✅ Answer: (A)
Question 38
What is an advantage of large scale monocultures?
(B) increased crop yield
(C) increased susceptibility to disease
(D) increased variation in the crop plant
▶️ Answer/Explanation
✅ Answer: (B)
Question 39
The graph shows the global emissions of carbon dioxide and methane from $1990$ to $2015$.

A student makes four statements about carbon dioxide and methane.
- Carbon dioxide and methane were the only sources of greenhouse gases produced in $1990$.
- Carbon dioxide emissions continue to rise to the present day.
- Carbon dioxide has a greater effect on climate change than methane.
- Methane emissions were lower than carbon dioxide emissions between $1990$ and $2015$.
How many statements are supported by these data?
(B) $2$
(C) $3$
(D) $4$
▶️ Answer/Explanation
1. The graph only shows $CO_{2}$ and methane; it does not prove they were the only gases produced.
2. The data ends at $2015$, so it cannot support a claim about the “present day” (e.g., $2026$).
3. The graph shows emission quantity, not the potency or effect on climate change.
4. Looking at the bars, the methane bars are significantly shorter than the carbon dioxide bars for every year shown ($1990–2015$).
Therefore, only the final statement is supported by the visual data provided.
✅ Answer: (A)
Question 40
The diagram shows a bacterium.
Which structure is cut to form sticky ends during genetic modification?

▶️ Answer/Explanation
✅ Answer: (C)
