Question 1

of the peripheral nervous system ……………………
that produces sweat ……………………
that requires energy for contraction. ……………………
Most-appropriate topic codes (Cambridge IGCSE Co-ordinated Sciences 0654, 2025–2027 syllabus):
• Topic B13.1 — Coordination and response (Part (a))
• Topic B13.3 — Homeostasis (Part (a)(ii)–(iii))
• Topic B13.2 — Hormones (Part (b))
• Topic B13.1 — Coordination and response (Part (c))
▶️ Answer/Explanation
(a)(i) peripheral nervous system = A; produces sweat = D; requires energy for contraction = B
A is a sensory nerve ending, part of the peripheral nervous system.
D is the sweat gland, which produces sweat.
B is the erector muscle, which requires energy (ATP) to contract.
(a)(ii) Vasodilation of the arteriole increases blood flow to the capillaries
Arterioles supplying the skin capillaries widen (vasodilate).
This increases blood flow to the capillaries close to the skin surface.
More heat is lost from the blood to the surroundings, cooling the body.
(a)(iii) Negative feedback
A change away from the set point triggers a response that reverses the change.
This return-to-normal mechanism is called negative feedback.
(b)(i) Insulin
Insulin is secreted by the pancreas when blood glucose concentration rises.
It causes glucose uptake by cells and conversion to glycogen, lowering blood glucose.
(b)(ii) Glands
Hormones are produced by (endocrine) glands.
These glands secrete hormones directly into the bloodstream.
(c)(i) Brain
The brain contains thermoreceptors.
These detect changes in the temperature of the blood flowing through it.
(c)(ii) Sensitivity
Sensitivity is the characteristic of living organisms involving detection of, and response to, stimuli.
Question 2



Most-appropriate topic codes (Cambridge IGCSE Co-ordinated Sciences 0654, 2025–2027 syllabus):
• Topic C1.1 — Solids, liquids and gases (Part (a))
• Topic C12.3 — Chromatography (Part (b))
• Topic C12.4 — Separation and purification (Part (c))
▶️ Answer/Explanation
(a)(i) liquid → gas = evaporation/boiling; liquid → solid = freezing/solidification
The forward arrow from liquid to gas represents a change of state called evaporation or boiling.
The reverse arrow from liquid to solid represents freezing (solidification).
(a)(ii) Kinetic energy increases
Heating transfers thermal energy to the gas particles.
This increases their kinetic energy, so they move faster.
(b)(i) Pencil marks are insoluble in the solvent (water)
Pencil (graphite) does not dissolve in the solvent used for chromatography.
Ink would dissolve and run up the paper with the solvent, distorting results.
(b)(ii) Dyes A, B and D
Comparing the spot positions of X with A, B, C and D on the chromatogram.
X matches the heights of spots A, B and D exactly, so these dyes are present in X.
(b)(iii) It is insoluble in the solvent
A substance that stays on the pencil line does not dissolve in the solvent.
Since it cannot move with the solvent front, it remains at the origin.
(b)(iv) \(R_f = 0.65\)
\( R_f = \dfrac{\text{distance moved by spot}}{\text{distance moved by solvent}} \)
\( R_f = \dfrac{2.6}{4.0} = 0.65 \)
(c) Solder melts over a range (220–229°C); tin and silver melt at a fixed point
Pure substances (tin, silver) have one specific, sharp melting point.
Mixtures (solder) melt over a range of temperatures because the different substances interfere with each other’s regular lattice arrangement.
Question 3

Calculate the speed of the athlete.
The gravitational field strength, \(g\), is 10 N/kg.


Most-appropriate topic codes (Cambridge IGCSE Co-ordinated Sciences 0654, 2025–2027 syllabus):
• Topic P1.5.1 — Effects of forces (Part (a)(i))
• Topic P1.6.1 — Energy (Part (a)(ii))
• Topic P1.2 — Motion (Part (b)(i)–(iii))
• Topic P1.5.2 — Turning effect of forces (Part (b)(iv))
• Topic P2.2.2 — Melting, boiling and evaporation (Part (c))
▶️ Answer/Explanation
(a)(i) Force A equals force B
Since the athlete swims at a constant speed, there is no resultant force.
So the forward force (B) and backward drag force (A) must be equal and opposite.
(a)(ii) speed = 0.6 m/s
Mass \(= \dfrac{750}{10} = 75\ \text{kg}\)
\(E_k = \tfrac{1}{2}mv^2 \Rightarrow v = \sqrt{\dfrac{2 \times 13.5}{75}} = 0.6\ \text{m/s}\)
(b)(i) 45 000 m ÷ 3600 s = 12.5 m/s
45 km/h is converted to m/s by dividing by 3600 and multiplying by 1000.
\( \dfrac{45\,000}{3600} = 12.5\ \text{m/s} \), confirming the given value.
(b)(ii) acceleration = 0.5 m/s²
\( a = \dfrac{\Delta v}{t} = \dfrac{12.5}{25} = 0.5\ \text{m/s}^2 \)
This is the gradient of the speed–time graph over the first 25 s.
(b)(iii) distance = 281.25 m
Area under the graph = triangle (0–25 s) + rectangle (25–35 s):
\( (\tfrac{1}{2} \times 25 \times 12.5) + (12.5 \times 10) = 156.25 + 125 = 281.25\ \text{m} \)
(b)(iv) force = 210 N
\( \text{Moment} = \text{force} \times \text{distance} \)
\( F = \dfrac{35.7}{0.17} = 210\ \text{N} \)
(c) Evaporation of the most energetic water molecules cools the skin
Thermal energy is transferred from the skin to the water molecules on its surface.
The most energetic molecules gain enough energy to escape as vapour (evaporate).
This lowers the average kinetic energy (temperature) of the remaining water and skin.
Question 4
Fig. 4.1 shows the apparatus she uses.

- places the lamp at 10 cm from the aquatic plant
- counts the number of oxygen bubbles released in 2 minutes
- repeats this two more times and calculates a mean
- repeats the process with the lamp at different distances from the aquatic plant.

Give your answer to the nearest whole number.
Write your answer in Table 4.1.
Suggest one reason for this difference.
Most-appropriate topic codes (Cambridge IGCSE Co-ordinated Sciences 0654, 2025–2027 syllabus):
• Topic B6.1 — Photosynthesis (Part (a)–(b))
• Topic B6.2 — Leaf structure (Part (c))
• Topic B6.1 — Photosynthesis, limiting factors (Part (d))
▶️ Answer/Explanation
(a) \(6CO_2 + 6H_2O \rightarrow C_6H_{12}O_6 + 6O_2\)
Carbon dioxide and water are the reactants of photosynthesis.
Glucose (\(C_6H_{12}O_6\)) is the organic product, alongside the given oxygen.
(b) Light energy → chemical energy
Chlorophyll absorbs light energy.
This is transferred into chemical energy stored in glucose.
(c) Palisade (mesophyll) cells
Palisade mesophyll cells are packed with chloroplasts.
Their position near the leaf’s upper surface maximises light absorption.
(d)(i) mean = 15
Mean \( = \dfrac{14+15+17}{3} = \dfrac{46}{3} = 15.33 \)
Rounded to the nearest whole number, mean = 15.
(d)(ii) As light intensity decreases, the rate of photosynthesis decreases, until it becomes constant
As the lamp distance increases (light intensity decreases), the mean number of bubbles/rate of photosynthesis decreases.
Beyond 40 cm, the rate becomes constant, showing light is no longer the limiting factor.
(d)(iii) Some of the oxygen produced is used in respiration
The plant respires continuously, consuming some of the oxygen it produces.
Only the surplus oxygen is released as bubbles, so less is measured than is actually produced.
Question 5

Fig. 5.2 shows the formation of a lithium ion, \(Li^+\), from a lithium atom.

Describe the lattice structure of ionic compounds.
You may include a labelled diagram if you wish.

Most-appropriate topic codes (Cambridge IGCSE Co-ordinated Sciences 0654, 2025–2027 syllabus):
• Topic C2.1 — Atomic structure (Part (a))
• Topic C2.4 — Ions and ionic bonds (Part (b))
• Topic C2.3 — Isotopes (Part (c))
▶️ Answer/Explanation
(a)(i) Nucleus contains protons and neutrons; outer particle is an electron
The central nucleus label should read “protons and neutrons.”
The particle orbiting in the outer shell is labelled “electron.”
(a)(ii) 2,1
Lithium has 3 electrons total.
These are arranged with 2 in the first shell and 1 in the second shell (2,1).
(b)(i) Chlorine atom gains one electron to form \(Cl^-\)
A chlorine atom (2,8,7) gains one electron from the lithium atom.
This forms a chloride ion with electronic structure (2,8,8) and a single negative charge, shown in square brackets with a “–” superscript.
(b)(ii) Regular arrangement of alternating positive and negative ions
Ionic lattices consist of oppositely charged ions arranged in a fixed, repeating (regular) 3D pattern.
Strong electrostatic forces of attraction hold the alternating positive and negative ions together.
(c)(i)
Carbon-13: 6 protons, 7 neutrons, 6 electrons.
Carbon-14: 6 protons, 8 neutrons, 6 electrons.
(Protons = atomic number = 6 for all isotopes; neutrons = mass number − protons.)
(c)(ii) Isotopes have the same number of electrons in the outer shell
Chemical properties depend on the arrangement of electrons, especially the outer shell.
Since all isotopes of carbon have the same number of electrons (and same outer shell arrangement), they react in the same way.
Question 6

Describe one environmental impact of using natural gas in this way.
The combustion of natural gas provides an input energy of 1.50 kJ.
Calculate the useful energy output from the boiler.
Describe the process of convection in terms of density changes.
Transverse waves are produced by vibrations acting ………………………………………………. to the direction of energy transfer.
Longitudinal waves are produced by vibrations acting ………………………………………………. to the direction of energy transfer.
An example of a longitudinal wave is a ………………………………………………. wave.
Most-appropriate topic codes (Cambridge IGCSE Co-ordinated Sciences 0654, 2025–2027 syllabus):
• Topic C10.2 — Air quality and climate (Part (a))
• Topic P1.6.4 — Power (Part (b))
• Topic P2.3.2 — Convection (Part (c))
• Topic P3.1 — General properties of waves (Part (d))
▶️ Answer/Explanation
(a) Contributes to global warming / enhanced greenhouse effect
Burning natural gas releases carbon dioxide.
This is a greenhouse gas that contributes to global warming/climate change.
(b) useful energy output = 1.35 kJ
\( \text{Output} = \text{efficiency} \times \text{input} \)
\( = 0.90 \times 1.50 = 1.35\ \text{kJ} \)
(c) Heated water becomes less dense and rises
Water near the flame is heated and expands, becoming less dense.
This less dense (hotter) water rises, while cooler, denser water sinks to take its place, setting up a convection current.
(d)(i) frequency \( \approx 6.5 \times 10^{14}\ \text{Hz}\)
Speed of light, \(c = 3 \times 10^8\ \text{m/s}\)
\( f = \dfrac{c}{\lambda} = \dfrac{3\times10^{8}}{4.6\times10^{-7}} \approx 6.5\times10^{14}\ \text{Hz} \)
(d)(ii) Transverse: perpendicular; Longitudinal: parallel; example: sound
In transverse waves, vibrations act perpendicular to the direction of energy transfer.
In longitudinal waves, vibrations act parallel to the direction of energy transfer.
Sound is a common example of a longitudinal wave.
Question 7

antibody production ……………………………………………………………………………………………………
blood clotting. ……………………………………………………………………………………………………………
Most-appropriate topic codes (Cambridge IGCSE Co-ordinated Sciences 0654, 2025–2027 syllabus):
• Topic B17.2 — Selection (Part (a))
• Topic B17.1 — Variation and mutation (Part (b))
• Topic B9.4 — Blood (Part (c))
▶️ Answer/Explanation
(a)(i) Non-resistant bacteria are killed by the antibiotic; resistant bacteria survive
The antibiotic kills the bacteria that do not have resistance.
The bacteria with the resistance allele/gene survive the treatment.
(a)(ii) Resistant bacteria multiply, passing on resistance to offspring
The surviving resistant bacteria reproduce (divide).
They pass on the resistance genes/DNA to their offspring, which are all resistant.
(a)(iii) Natural selection
This process, where organisms best suited to their environment survive and reproduce, is called natural selection.
(b)(i) A mutation is a change in a gene or chromosome
A mutation is a random change in the DNA sequence of a gene or the structure/number of a chromosome.
(b)(ii) Ionising radiation
Ionising radiation (e.g. UV, X-rays, gamma rays) can damage DNA, increasing the rate of mutation.
(c) antibody production = white blood cells; blood clotting = platelets
White blood cells (lymphocytes) produce antibodies as part of the immune response.
Platelets are responsible for triggering blood clotting at a wound.
Question 8

The same volume and concentration of hydrochloric acid and the same mass of magnesium ribbon are used in each experiment.
She measures the time for the magnesium to completely react at each temperature.
Table 8.1 shows her results.

Explain how you can tell this from Table 8.1.

Use the axes shown in Fig. 8.2 to draw and label the energy level diagram for this reaction.
- the energy of the reactants and the products
- the energy change in the reaction
- the activation energy of the reaction.

Calculate the volume occupied by 0.1 g of hydrogen gas.
The volume of one mole of any gas is \(24\ \text{dm}^3\) at room temperature and pressure (r.t.p.).
Most-appropriate topic codes (Cambridge IGCSE Co-ordinated Sciences 0654, 2025–2027 syllabus):
• Topic C9.4 — Reactivity series (Part (a))
• Topic C6.2 — Rate of reaction (Part (b))
• Topic C5.1 — Exothermic and endothermic reactions (Part (c))
• Topic C3.3 — The mole and the Avogadro constant (Part (d))
▶️ Answer/Explanation
(a) \( Mg + 2HCl \rightarrow MgCl_2 + H_2 \)
Magnesium displaces hydrogen from hydrochloric acid.
Formulae and coefficients must balance atoms on both sides.
(b)(i) The time decreases as temperature increases
Table 8.1 shows the time taken falls steadily from 119 s (20°C) to 31 s (40°C).
A shorter time for the same amount of reactant to react means a faster rate.
(b)(ii)
Higher temperature gives particles more kinetic energy, so they move faster.
Faster-moving particles collide more frequently and with greater force, increasing the reaction rate.
(c) 
For an exothermic reaction, the products’ energy level is lower than the reactants’.
The energy change (ΔH) is the vertical drop from reactants to products.
The activation energy is the “hump” from reactants up to the peak of the curve.
(d) volume = 1.2 dm³
\( M_r \) of \(H_2 = 2\)
Moles \( = \dfrac{0.1}{2} = 0.05\ \text{mol}\)
Volume \( = 0.05 \times 24 = 1.2\ \text{dm}^3 \)
Question 9

She measures this length with the ruler and uses the ammeter reading to calculate the resistance of the wire.
When the wire is made longer, the reading on the ammeter decreases.
Explain why the reading on the ammeter decreases.

The student records the potential difference across the wire and the current in the wire.
Fig. 9.3 shows her results.

State the meaning of the term electromotive force (e.m.f.).

Most-appropriate topic codes (Cambridge IGCSE Co-ordinated Sciences 0654, 2025–2027 syllabus):
• Topic P4.2.4 — Resistance (Part (a))
• Topic P4.3.1 — Circuit diagrams and circuit components (Part (b)(i))
• Topic P4.2.4 — Resistance, I–V graphs (Part (b)(ii))
• Topic P4.2.3 — Voltage (electromotive force and potential difference) (Part (c))
• Topic P4.5.3 — Magnetic effect of current (Part (d))
▶️ Answer/Explanation
(a) A longer wire has greater resistance, so current decreases
Resistance of a wire increases as its length increases.
Since current is inversely proportional to resistance (for a fixed e.m.f.), the ammeter reading decreases.
(b)(i) Variable resistor
Component X, drawn with an arrow through a rectangle, is a variable resistor (rheostat), used to change the current/p.d. in the circuit.
(b)(ii) resistance ≈ 1.9 Ω
\( R = \dfrac{V}{I} \)
Using the graph, at \(I = 0.80\ \text{A}\), \(V = 1.50\ \text{V}\).
\( R = \dfrac{1.50}{0.80} \approx 1.9\ \Omega \)
(c) e.m.f. is the energy supplied (work done) per unit charge driven around a complete circuit
Electromotive force is the electrical work done by a source in driving charge around a complete circuit, per unit charge.
(d) Concentric circles around the wire, direction shown
The magnetic field forms concentric circles centred on the wire.
Its direction is found using the right-hand grip rule, based on the direction of current flow.
Question 10

The concentrated salt solution has a lower ………………………………………………. than cell A.
Water crosses the ………………………………………………. and leaves the cell by osmosis.
Water molecules move from a more ………………………………………………. solution to a more ………………………………………………. solution.
Suggest how this change in shape affects the function of red blood cells in the body.
Suggest two other factors that affect the rate of osmosis.
Most-appropriate topic codes (Cambridge IGCSE Co-ordinated Sciences 0654, 2025–2027 syllabus):
• Topic B3.2 — Osmosis (Part (a), (c))
• Topic B9.4 — Blood (Part (b))
• Topic B2.1 — Cell structure (Part (d))
▶️ Answer/Explanation
(a) lower water potential; crosses the membrane; more dilute to more concentrated
The salt solution has a lower water potential than the cell.
Water leaves the cell across the (partially permeable) cell membrane by osmosis.
Water moves from a more dilute solution (inside the cell) to a more concentrated solution (outside).
(b) Reduced surface area/volume means less oxygen can be transported
The shrunken, spiky shape reduces the cell’s surface area available for gas exchange.
This means less oxygen can be carried/transported around the body.
(c) Surface area and temperature
A larger surface area increases the rate of osmosis.
Higher temperature increases the kinetic energy of water molecules, increasing the rate.
(d)(i) Chloroplast and (permanent) vacuole (or cell wall)
Plant cells contain chloroplasts (for photosynthesis) and a permanent vacuole, structures not found in animal cells.
A cellulose cell wall is also unique to plant cells.
(d)(ii) Root hair cell
Root hair cells have a large surface area, specialised for the absorption of water (and mineral ions) from the soil.
Question 11

Nylon is made in a condensation polymerisation reaction.

Use the information in Fig. 11.2 in your answer.
Most-appropriate topic codes (Cambridge IGCSE Co-ordinated Sciences 0654, 2025–2027 syllabus):
• Topic C11.5 — Alkenes (Part (a))
• Topic C11.7 — Polymers (Part (b))
▶️ Answer/Explanation
(a)(i) B
Catalytic addition of steam to an alkene (A, ethene) produces an alcohol.
Compound B is ethanol.
(a)(ii) C
Addition of hydrogen to an alkene produces the corresponding alkane.
Compound C is ethane.
(a)(iii) A
Compound A (ethene, \(C_2H_4\)) reacts with bromine by addition across the C=C double bond.
This forms \(C_2H_4Br_2\) (1,2-dibromoethane).
(a)(iv) A
Compound E is a repeating polymer unit derived from a \(C=C\) monomer.
Compound A (ethene) undergoes addition polymerisation to form this polymer (polyethene).
(b) Monomer X and Y join, losing a small molecule (water)
The carbon (–COOH) group of monomer X joins with the nitrogen (–NH) group of monomer Y.
An –OH is lost from monomer X and an –H is lost from monomer Y.
These combine to eliminate a molecule of water, forming the amide (C–N) link.
Question 12

Table 12.1 shows the student’s results.

Calculate the time it will take for the activity of the source to drop to 12.5% of the original value.
The melting point of lead is 327°C. When lead melts, it turns from a solid into a liquid.
Describe the changes in the forces between particles when a solid melts.
A sample of liquid lead has a mass of 37.1 g.
Calculate the volume of the sample of liquid lead.
Most-appropriate topic codes (Cambridge IGCSE Co-ordinated Sciences 0654, 2025–2027 syllabus):
• Topic P5.2.2 — The three types of nuclear emission (Part (a)(i))
• Topic P5.2.4 — Half-life (Part (a)(ii))
• Topic P2.2.1 — Thermal expansion of solids, liquids and gases (Part (b))
• Topic P1.4 — Density (Part (c))
▶️ Answer/Explanation
(a)(i) Beta radiation
The count rate stays high through paper but drops sharply once thin aluminium is added, then changes little afterwards.
This shows the radiation can penetrate air and paper, but not thin aluminium — the pattern characteristic of beta particles.
(a)(ii) time = 87 years
12.5% remaining corresponds to \( \left(\dfrac{1}{2}\right)^3 \), i.e. 3 half-lives.
Time \( = 3 \times 29 = 87\) years.
(b) The forces between particles decrease
As a solid melts, the strong forces holding particles in fixed positions weaken.
This allows particles to move more freely past one another, forming a liquid.
(c) volume = 3.5 cm³
\( V = \dfrac{m}{d} \)
\( V = \dfrac{37.1}{10.6} \approx 3.5\ \text{cm}^3 \)
