Home / IB DP Biology D1.2 Protein synthesis-FA 2025- IB Style Questions For SL Paper 2

IB DP Biology D1.2 Protein synthesis-FA 2025- IB Style Questions For SL Paper 2

Question

(a) Outline the structure of proteins.

(b) Cells produce a large variety of proteins with different sequences of amino acids. Explain how this is done.

(c) Outline the range of functions of proteins in cells.

▶️ Answer/Explanation

(a) 

  • Proteins are made up of building blocks called amino acids.
  • There are about 20 different amino acids used in making proteins.
  • The amino acids are joined together by peptide bonds to form chains called polypeptides.
  • A protein might be made of one or more polypeptide chains.
  • These chains fold into a specific 3D shape, and that shape is what gives the protein its job or function.

(b)

  • A gene in the DNA gives the instructions to make a protein – it’s like a recipe.
  • When a gene is “turned on,” it goes through a process called transcription, where the DNA is copied into mRNA.
  • The mRNA leaves the nucleus and goes to a ribosome, which is the protein-making machine.
  • At the ribosome, translation happens: the mRNA code is read in groups of three bases (called codons), and each codon tells the ribosome which amino acid to add.
  • tRNA molecules bring the correct amino acids to the ribosome.
  • The amino acids are linked together in the right order to form a polypeptide.
  • The sequence of amino acids depends on the order of bases in the DNA, and since each gene has a different sequence, it makes a different protein.
  • Also, mutations (small changes in DNA) can change the protein, adding even more variety.

(c)

  • Enzymes – speed up chemical reactions, like breaking down food or building molecules.
  • Hormones – some proteins like insulin act as messengers between cells.
  • Transport proteins – help move things across cell membranes (like glucose or ions).
  • Hemoglobin – a protein in red blood cells that carries oxygen around the body.
  • Receptors and cell markers – found on cell membranes, they help cells communicate and recognize each other.
  • Structural proteins – like actin and myosin in muscles that help with movement, or proteins that form spindle fibers during cell division.
  • Histones – help organize and pack DNA tightly into chromosomes.

Markscheme

a

a. protein formed from amino acids OR 20 different amino acids
b. linked together by peptide bonds
c. may consist of one or more polypeptides linked together
d. have a specific shape/conformation/folding
e. shape determines function

b

a. protein is produced when a gene is expressed/switched on
b. genetic code/codons consists of three nucleotides/bases/base triplet
c. genetic code in DNA is transcribed/transcription (to mRNA)
d. mRNA exits the nucleus
e. mRNA (code) is translated/translation into a polypeptide/protein
f. amino acid sequence/polypeptide formation occurs at a ribosome
g. one codon translates to one amino acid
h. tRNA carries code for specific amino acids
i. tRNA anticodon matches with specific codon in mRNA
j. amino acids joined (by peptide bonds) to form polypeptide
k. sequence of amino acids determined by order of bases/nucleotides/codons in DNA/mRNA
l. proteins vary based on which amino acids are used and their order OR protein variety increases by mutations to DNA

c

a. enzymes that catalyse/speed up/control (the rate and direction of) metabolic reactions
b. proteins can be hormones which are chemical messengers to cells
c. proteins that transport through the membrane such as channel/carrier/pumps / that regulate what enters/leaves the cell
d. hemoglobin in red blood cells that transports/binds oxygen
e. membrane proteins for cell/tissue recognition/cell adhesion/communication
f. structural elements of muscle fibre/actin/myosin for movement OR spindle fibres move chromosomes
g. histones condense DNA into chromosomes

Question

The diagram shows the molecular structure of the amino acid leucine.

Leucine molecular structure

(a) Draw a circle on the diagram to enclose the carboxyl group.

(b) State one protein that acts as a hormone.

(c) Explain how enzymes are used in the preparation of milk suitable for individuals who are lactose intolerant.

▶️ Answer/Explanation

(a)

Leucine with circled carboxyl group

(b)  Insulin

(c) 

People with lactose intolerance can’t properly digest lactose, the sugar found in milk. Here’s how enzymes help:

  1. Lactase is the enzyme used—it breaks down lactose.
  2. The lactase enzyme is immobilized—meaning it’s stuck to tiny beads (often made from alginate, a jelly-like material).
  3. When milk is poured over or mixed with these beads, the lactase breaks down the lactose into two simpler sugars:
    glucose and galactose
    These are easier to digest and don’t cause symptoms.

 

Markscheme

a

Leucine with circled carboxyl group

b

insulin/glucagon/ADH/oxytocin/leptin/other verified example

c

a. the enzyme lactase acts on lactose
b. the enzymes are immobilized/are bound to alginate beads
c. (pouring milk over the immobilized enzymes) causes lactose to be hydrolyzed/broken down into monosaccharides/glucose and galactose

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