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IB DP Biology Gas exchange Study Notes | IB DP Biology 2025

IB DP Biology Gas exchange Study Notes - New Syllabus

IB DP Biology Gas exchange Study Notes

IB DP Biology Gas exchange Study Notes Study Notes at  IITian Academy  focus on  specific topic and type of questions asked in actual exam. Study Notes focus on IB Biology syllabus with guiding questions of

  • How are multicellular organisms adapted to carry out gas exchange?
  • What are the similarities and differences in gas exchange between a flowering plant and a mammal?

Standard level and higher level: 3 hours
Additional higher level: 1 hour

IBDP Biology 2025 -Study Notes -All Topics

B3.1.1 – Gas Exchange as a Vital Function in All Organisms

Gas Exchange = Oxygen In, CO₂ Out
Needed for respiration
Small organisms: Diffusion
Large organisms: Specialized systems

All living organisms need energy to perform essential functions like movement, growth, and repair. This energy is released through aerobic respiration, which requires oxygen and produces carbon dioxide as a waste product.

Glucose + Oxygen ⟶ Carbon dioxide + Water + Energy (ATP)

Gas exchange is the biological process by which oxygen is taken in and carbon dioxide is removed. In most organisms, this happens by diffusion across membranes or specialized surfaces.

Gas Exchange in Different Organisms

  • Plants: Take in CO₂ for photosynthesis and release O₂. They also respire all the time, using O₂ and producing CO₂. Gases move through stomata on leaves.
  • Unicellular organisms: Use their whole-body surface for gas exchange due to a high surface area-to-volume ratio.

Surface Area-to-Volume Ratio (SA:V)

Organism SizeSA:V RatioGas Exchange Efficiency
SmallLargeEfficient by diffusion
LargeSmallNeeds specialized systems

As organisms grow, their volume increases faster than surface area, reducing their SA:V ratio. This makes diffusion alone too slow for effective gas exchange.

Why Specialized Systems Are Needed

Larger organisms have internal cells that are far from the external environment. They need:

  • Special exchange surfaces (lungs, gills, tracheae)
  • Transport systems (blood, haemolymph)
Adaptations for gas exchange: large surface area, thin barriers, moist surfaces, and good blood supply or ventilation.

Why the need for a gas exchange system inside large organisms? 

   

   

Summary

  • Gas exchange is essential for aerobic respiration in all organisms.
  • Small organisms use simple diffusion due to high SA:V ratios.
  • Larger organisms need specialized systems to ensure efficient gas exchange over longer distances.

B3.1.2 – Properties of Gas Exchange Surfaces

4 Key Features of Gas Exchange Surfaces:
Thin • Permeable • Moist • Large surface area

To ensure efficient gas exchange, all organisms have evolved specialized surfaces that allow oxygen to enter and carbon dioxide to exit rapidly. Despite structural differences, these surfaces share four essential features:

1. Thin Tissue Layer

Gas exchange surfaces are typically only one cell thick, minimizing the diffusion distance and speeding up gas exchange.

Example: In human lungs, alveolar and capillary walls together are less than 1 μm thick.

2. Permeability

Gas exchange surfaces must allow oxygen and carbon dioxide to diffuse freely. These gases are small and nonpolar, so they move easily through cell membranes by simple diffusion.

No barriers = faster diffusion

3. Moisture

Gases must dissolve in water before they can diffuse across membranes. That’s why gas exchange surfaces are always kept moist.

Moisture allows efficient diffusion by dissolving gases before they move across cell layers.

4. Large Surface Area

A larger surface area increases the amount of gas that can diffuse at any one time, compensating for the naturally slow process of diffusion.

Example: Human lungs have millions of alveoli, giving a surface area of ~70 m² – about the size of a tennis court!

Summary of Key Features

FeatureFunction
Thin surfaceShort diffusion distance ⟶ faster gas exchange
PermeableAllows O₂ and CO₂ to diffuse freely
MoistEnables gases to dissolve before diffusing
Large surface areaMaximizes total gas exchange capacity

 

B3.1.3 – Maintenance of Concentration Gradients at Exchange Surfaces in Animals

Why are gradients important?
Gas exchange only works efficiently if a steep concentration gradient is maintained between oxygen and carbon dioxide.

Oxygen and carbon dioxide move by diffusion. For diffusion to stay rapid and effective, there must be a constant concentration gradient across the gas exchange surface.

1. Dense Network of Blood Vessels

Capillaries are packed closely around the alveoli (lungs) or gills (fish). They quickly:

  • Carry away oxygen after it diffuses in
  • Deliver carbon dioxide from body tissues
This ensures blood near the surface is always low in O₂ and high in CO₂ – keeping the gradient steep.

2. Continuous Blood Flow

Blood is constantly moving, which prevents buildup of oxygen or slow removal of carbon dioxide.

Fresh, deoxygenated blood keeps arriving at the exchange surface, maintaining a high diffusion rate.

3. Ventilation (Air or Water Movement)

Ventilation ensures that the external side of the surface (air or water) stays high in O₂ and low in CO₂:

  • In lungs → breathing brings in fresh oxygen-rich air
  • In gills → water flows over the surface constantly
Ventilation keeps the gradient steep across the external environment too.

🔑 Summary of Gradient Maintenance

MechanismFunction
Dense capillary networkQuickly removes O₂ and brings CO₂ ⟶ keeps blood side steep
Continuous blood flowDelivers fresh blood ⟶ prevents oxygen saturation
VentilationMaintains external O₂/CO₂ levels ⟶ gradient across the surface

 

B3.1.4 – Adaptations of Mammalian Lungs for Gas Exchange

Why is gas exchange vital?
✔ Oxygen fuels aerobic respiration
✔ CO₂ must be removed to avoid toxicity and maintain pH

The lungs are adapted to allow rapid and efficient exchange of gases between air and blood. Several structural features support this essential function.

                                                   

1. Alveoli – The Main Site of Gas Exchange

Alveoli are tiny air sacs found at the end of bronchioles. Each lung has ~300 million alveoli, providing a vast surface area for diffusion.

Their round, folded structure maximizes surface area, enhancing the rate of gas exchange.

2. Very Thin Walls

Alveolar and capillary walls are one cell thick, reducing the diffusion distance to less than 1 µm.

Short diffusion distance = faster gas exchange

3. Surfactant Production

Type II pneumocytes inside alveoli secrete surfactant – a fatty fluid that reduces surface tension.

  • Prevents alveoli from collapsing
  • Keeps alveoli moist for efficient gas diffusion

4. Dense Network of Capillaries

Each alveolus is wrapped in a rich bed of capillaries, which:

  • Deliver CO₂ from tissues
  • Carry away O₂ to the body
Maintains steep O₂ and CO₂ gradients for constant diffusion

5. Highly Branched Bronchial Tree

Air enters the lungs through a system of branching tubes:

Trachea → Bronchi → Bronchioles → Alveoli

This branching structure increases surface area and ensures air reaches deep into the lungs.

6. Ventilation Maintains Gradients

Breathing movements bring in fresh air and remove used air:

  • Inhalation: Brings in O₂-rich air
  • Exhalation: Removes CO₂-rich air
Keeps gas concentrations optimal on the alveolar side

✅ Summary of Lung Adaptations

AdaptationFunction
Thin wallsShort diffusion distance
Large surface area (alveoli)More gas exchange per breath
Rich capillary networkMaintains steep gradients
SurfactantPrevents collapse & helps diffusion
VentilationRefreshes air to maintain gradients

 

B3.1.5 – Ventilation of the Lungs

Ventilation = Breathing
Inhalation: breathing in
Exhalation: breathing out
Maintains steep O₂ and CO₂ gradients for efficient gas exchange

Ventilation is the physical movement of air into and out of the lungs. It involves two processes:

  • Inhalation (Inspiration): Brings in fresh air rich in oxygen
  • Exhalation (Expiration): Removes air rich in carbon dioxide

🫧 Inhalation (Breathing In)

During inhalation:

  • Diaphragm contracts and moves downward (flattens)
  • External intercostal muscles contract → ribs move up & out
  • Thoracic cavity volume increases
  • Pressure inside lungs drops below atmospheric pressure
  • Air flows into the lungs
✅ Inhalation is an active process – it requires energy.

 

💨 Exhalation (Breathing Out)

During exhalation:

  • Diaphragm relaxes and moves upward (dome shape)
  • External intercostal muscles relax → ribs move down & in
  • Abdominal muscles may contract (especially during forced exhalation)
  • Thoracic cavity volume decreases
  • Pressure inside lungs increases above atmospheric pressure
  • Air flows out of the lungs
✅ Normal exhalation is passive but becomes active during exercise or forced breathing.

🧠 Summary: Muscles Involved in Ventilation

Muscle GroupDuring InhalationDuring Exhalation
DiaphragmContracts ↓Relaxes ↑
External IntercostalsContract ↑ & →Relax ↓ & ←
RibsMove up and outMove down and in
Abdominal MusclesRelaxMay contract

 

B3.1.6 – Measurement of Lung Volumes

Vital Capacity = TV + IRV + ERV

✅ You can measure:
– Tidal Volume (TV)
– Inspiratory Reserve (IRV)
– Expiratory Reserve (ERV)

❌ You can’t directly measure:
– Residual Volume (RV)
– Total Lung Capacity (TLC)

📊 Key Lung Volume Terms

TermDescription
Tidal Volume (TV)Air breathed in or out in a normal breath (at rest)
Inspiratory Reserve (IRV)Extra air you can forcefully inhale after a normal breath
Expiratory Reserve (ERV)Extra air you can forcefully exhale after a normal breath
Vital Capacity (VC)Maximum air exhaled after a full inhalation (TV + IRV + ERV)
Residual Volume (RV)Air left in lungs after forced exhalation (not measurable directly)
Total Lung Capacity (TLC)Vital Capacity + Residual Volume

🧪 How Do We Measure These?

Using a spirometer: a machine that records breathing patterns on a graph.

  • Breathe normally → record Tidal Volume (TV)
  • Inhale deeply → record Inspiratory Reserve (IRV)
  • Exhale forcefully → record Expiratory Reserve (ERV)
  • Calculate Vital Capacity = TV + IRV + ERV
Spirometry Graph: Wavy line that rises/falls with breathing. Peaks show inhalation; dips show exhalation.

📐 Example Values (Average Adult)

  • Tidal Volume (TV): ~500 mL
  • Inspiratory Reserve (IRV): ~3000 mL
  • Expiratory Reserve (ERV): ~1100 mL
  • Vital Capacity (VC): ~4600 mL
🧠 Note: Values vary depending on age, gender, fitness, and health.

B3.1.7 – Adaptations for Gas Exchange in Leaves

📌 Leaf Features

– Large surface area → more air contact
– Thin → short diffusion distance
– Air spaces → fast gas movement
– Stomata + guard cells → regulate flow
– Moist inner surface → gases dissolve
– Veins → water in, sugars out

Leaves are adapted for efficient gas exchange, which is essential for photosynthesis and respiration.

🌬️ Why Do Leaves Need Gas Exchange?

  • Photosynthesis needs CO₂ and releases O₂
  • Respiration (in all cells) uses O₂ and produces CO₂
  • Leaves must allow gas exchange without losing too much water

🧬 Key Adaptations in Leaf Structure

StructureFunction (How it helps gas exchange)
Waxy CuticleThin, waterproof layer that reduces water loss but allows light through
Upper EpidermisTransparent, protective layer that lets light reach photosynthetic cells
Spongy MesophyllLoosely packed cells with air spaces to allow easy gas diffusion
Air SpacesAllow gases like CO₂ and O₂ to diffuse quickly between cells and stomata
Stomata (pores)Let CO₂ enter and O₂ exit the leaf
Guard CellsSurround each stoma and control opening/closing
Veins (xylem & phloem)Xylem brings water; Phloem carries sugars away

🌡️ How Do Guard Cells Work?

  • Location: Mostly on underside of the leaf
  • Only epidermal cells with chloroplasts

Daytime (light):

  • Photosynthesis → sugar made
  • Guard cells absorb K⁺ ions
  • Water enters by osmosis → cells swell
  • Stoma opens to let CO₂ in

Night / Water stress:

  • K⁺ pumped out → water exits
  • Guard cells shrink → stoma closes
✅ Guard cells regulate gas exchange and minimize water loss – a key adaptation for survival.

B3.1.8 – Distribution of Tissues in a Leaf

📚 Basic Structure of a Dicot Leaf (Transverse Section)

Below is a simplified order of tissue layers from top to bottom:

  • Cuticle
  • Upper Epidermis
  • Palisade Mesophyll
  • Spongy Mesophyll
  • Lower Epidermis (with stomata and guard cells)
  • Cuticle

🔬 Tissue Distribution & Functions

Tissue / LayerKey Features & Function
CuticleWaxy outer layer (top and bottom)
Prevents water loss
Upper EpidermisTransparent cells
Protect internal tissues from damage & microbes
Palisade MesophyllTightly packed, column-shaped cells
Rich in chloroplasts → main site of photosynthesis
Spongy MesophyllLoosely packed with air spaces
Allows gas diffusion between cells
Vein (Vascular Bundle)Contains xylem (for water) & phloem (for sugars)
Leaf’s transport system
Lower EpidermisHas stomata and guard cells
Regulates gas exchange and water loss
Guard CellsBean-shaped, contain chloroplasts
Open/close stomata for gas control
📌 Summary: Leaf tissues are perfectly arranged to maximize light absorption, gas exchange, and transport – making the leaf highly efficient for photosynthesis and survival.

B3.1.9 – Transpiration as a Consequence of Gas Exchange in a Leaf

🌵 Xerophytes → adapted to dry (arid) habitats
Halophytes → adapted to salty environments

Both minimize water loss through unique structural & physiological features.

Transpiration is the loss of water vapor from the surface of leaves, mainly through the stomata during gas exchange.

                                             

This process is an unavoidable side-effect of stomata opening to allow carbon dioxide in for photosynthesis. When water potential (Ψ) inside the leaf is higher than the surrounding air, water vapor exits.

🌞 When Does It Happen?

  • Mostly during the daytime when stomata are open
  • Nighttime transpiration is minimal (stomata are usually closed)
  • Plants can lose up to 98% of absorbed water through transpiration!

 

📊 Factors Affecting Rate of Transpiration

FactorEffect on Transpiration
Light IntensityMore light → stomata open wider → more water loss
TemperatureWarmer air holds more water vapor → increases evaporation rate
HumidityHigh humidity → reduces water potential gradient → slows transpiration
Wind / Air MovementWind removes humid air near stomata → maintains steep gradient
Soil Water AvailabilityDry soil → limits water uptake → reduces transpiration

🌱 Link with Gas Exchange

Stomata open to allow CO₂ to enter for photosynthesis. At the same time, oxygen and water vapor exit the leaf. Thus, transpiration and gas exchange are directly linked.

📌 Summary: Transpiration is a side-effect of photosynthetic gas exchange. It helps with cooling, nutrient transport, and water movement, but excessive loss can cause wilting. Understanding its regulation is vital for plant survival.

B3.1.10 – Stomatal Density

Stomatal density is the number of stomata per mm² of leaf surface. These pores regulate gas exchange and water loss.

More stomata = greater potential for photosynthesis and transpiration.

🧪 How Do We Measure It?

  • Leaf Casts: Apply clear nail polish to leaf, peel it off, and place it under a microscope.
  • Micrographs: Take clear images of the leaf surface and count stomata using a grid or scale.

📏 Steps for Accurate Measurement

  • Select a known field of view under high magnification.
  • Count stomata in that area.
  • Repeat for multiple areas on the same leaf.
  • Calculate the average number of stomata per mm².

 

🔍 Why Repeat Counts?
Biological samples are variable
→ Stomatal number may vary across a single leaf
→ Repeating measurements gives more reliable, unbiased data
→ Always calculate an average from multiple fields of view

🌿 Link to Environmental Conditions

EnvironmentStomatal Density Trend
Dry ConditionsFewer stomata to reduce water loss
Humid ConditionsMore stomata to allow maximum gas exchange
📌 Summary: Stomatal density reflects how well a plant is adapted to its environment. Practical measurement reinforces key investigation skills—especially the importance of repeating and averaging data.

Additional Higher Level

B3.1.11 – Adaptations of Foetal and Adult Haemoglobin for Oxygen Transport

🧠 Tip:
Foetal Hb → left shift → higher O₂ affinity
Bohr effect → right shift → more O₂ unloading in active tissues

Haemoglobin (Hb) is the protein in red blood cells that carries oxygen. It’s made of 4 polypeptide chains and 4 haem groups, each binding one molecule of oxygen.

🧷 Cooperative Binding of Oxygen

When the first O₂ molecule binds, it causes a conformational change, making it easier for the next three to bind. This is called cooperative binding, and it leads to an S-shaped (sigmoidal) oxygen dissociation curve.

Foetal vs. Adult Haemoglobin

FeatureFoetal HbAdult Hb
Oxygen affinityHigherLower
ReasonTakes O₂ from maternal bloodAlready exposed to high O₂ in lungs
Dissociation curveShifted to the leftNormal or slightly right-shifted

👉 Why left shift for foetus?
Higher O₂ affinity → binds oxygen more readily in placenta, even at low O₂ concentration.

The Bohr Effect – CO₂’s Role

CO₂ binds to haemoglobin at a different site (not the haem group). This is allosteric binding, which changes Hb’s shape and reduces its oxygen affinity.

High CO₂ → more H⁺ ions → more acidic → promotes O₂ unloading in tissues. This is known as the Bohr Shift – the oxygen dissociation curve shifts to the right.

🔁 Summary of Adaptations

AdaptationPurpose
Cooperative bindingEfficient oxygen loading in lungs
Foetal Hb higher affinityTakes O₂ from mother’s blood
Bohr Effect (CO₂ binding)Promotes O₂ unloading where it’s needed

B3.1.12 – Bohr Shift

🧠 Tip:

Right shift = Release of O₂
Bohr shift helps muscles get more oxygen during exercise or stress!

The Bohr Shift explains how increased carbon dioxide (CO₂) in tissues causes haemoglobin (Hb) to release more oxygen (O₂). This effect is crucial in active tissues that produce lots of CO₂, such as during exercise.

🔁 How It Works (Step-by-Step)

  1. Active cells respire → produce more CO₂.
  2.  CO₂ dissolves in blood and reacts with water to form carbonic acid (H₂CO₃):
    CO₂ + H₂O→ H₂CO₃
  3. Carbonic acid releases H⁺ ions → lowers blood pH.
  4. H⁺ binds to haemoglobin → changes its shape.
  5. This reduces Hb’s affinity for oxygen → O₂ is released more easily.

📊 Effect on the Oxygen Dissociation Curve

The curve shifts to the right
More O₂ is unloaded at higher CO₂ levels
Tissues get more oxygen when they need it most

💪 Benefits of the Bohr Shift

FeatureBenefit to Body
More O₂ released in active tissuesSupports high respiration rate
Responds to ↑ CO₂ and ↓ pHHelps maintain homeostasis
Curve shifts rightEfficient O₂ delivery under stress

🧪 Summary

More CO₂ → More H⁺ → Lower pH → Hb releases O₂

The Bohr effect allows faster and more targeted oxygen delivery to where it’s needed most – like working muscles and organs. 

B3.1.13 – Oxygen Dissociation Curves & Haemoglobin’s Affinity for Oxygen

💡Tip:

Cooperative binding makes oxygen load quickly in lungs and unload fast in tissues.

The sigmoid curve reflects haemoglobin’s smart oxygen delivery!

The Oxygen Dissociation Curve is a graph that shows how much oxygen (O₂) is bound to haemoglobin (Hb) at different oxygen pressures.

  • Y-axis: % saturation of haemoglobin with O₂
  • X-axis: Partial pressure of oxygen (pO₂)

🧬 Why is the Curve S-Shaped (Sigmoidal)?

  • Cooperative binding causes the S-shape.
  • Binding of first O₂ makes it easier for the next ones.
  • Curve steepens in middle, then flattens at top as Hb gets saturated.

Haemoglobin has 4 haem groups. As each O₂ binds, it alters the shape of Hb slightly, increasing its affinity for the next O₂ molecule. However, once nearly full, it becomes harder for the last O₂ to bind – causing the curve to plateau.

In the Lungs: High Affinity

At high pO₂ (in lungs), Hb has high oxygen affinity and binds O₂ easily, forming oxyhaemoglobin (HbO₂). Blood becomes highly saturated with oxygen.

In Tissues: Low Affinity

At low pO₂ (in tissues), Hb’s affinity drops, causing it to release oxygen where it’s needed for respiration.

🔄 Cooperative Binding Summary

Binding StageAffinity for O₂Curve Behavior
1st O₂LowCurve starts shallow
2nd & 3rd O₂HigherCurve steepens
4th O₂Lower againCurve levels off

Haemoglobin structure

Haemoglobin = Respiratory pigment

  • Haemoglobin has quaternary structure
  • Four subunits
  • Two alpha chains
  • Two beta chains
  • Each contains a haeme group that can bind oxygen

 

Haemoglobin binding oxygen

📊 What the Curve Tells Us

Steep middle section: Small pO₂ change → Large O₂ release
Right shift: Hb releases more O₂ (e.g. Bohr shift)
Left shift: Hb holds onto O₂ more (e.g. fetal haemoglobin)
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