Home / iGCSE Biology (0610)-21.1 Biotechnology and genetic modification- iGCSE Style Questions Paper 3

iGCSE Biology (0610)-21.1 Biotechnology and genetic modification- iGCSE Style Questions Paper 3

Question

(a) Yoghurt can be made by adding the bacteria Lactobacillus bulgaricus to milk.
(i) State the genus name for these bacteria.
(ii) State two reasons why bacteria are useful in biotechnology processes such as making yoghurt.

(b) Fig. 6.1 shows the nutritional information for $100\text{ g}$ of a type of yoghurt.

(i) A person wants to eat $45\text{ g}$ of protein.
The person ate $200\text{ g}$ of yoghurt.
Using the information in Fig. 6.1, calculate the mass of protein the person needs to eat, in addition to the yoghurt, to reach $45\text{ g}$ of protein.
(ii) State the importance of fats in the diet.
(iii) Yoghurt does not contain fibre. State one principal dietary source of fibre.
(c) Yeast is another microorganism that is used in biotechnology. Anaerobic respiration in yeast is used in bread-making.
(i) State the word equation for anaerobic respiration in yeast.
(ii) Describe the role of anaerobic respiration in yeast during bread-making.
(iii) State one other use of yeast in biotechnology.
▶️ Answer/Explanation
Detailed solution & Explanation

6(a)(i) Genus: Lactobacillus
Explanation: In binomial nomenclature, the first name is the Genus (capitalized) and the second is the species.

6(a)(ii) Usefulness: Rapid reproduction rate; ability to make complex molecules (e.g., enzymes/acids).
Explanation: Bacteria are ideal for biotechnology because they grow extremely fast under the right conditions and have simple genetic structures that allow them to produce specific chemicals, like lactic acid, which thickens milk into yoghurt.

6(b)(i) Calculation:
Mass of protein in $200\text{ g}$ of yoghurt: $7\text{ g}$ ($3.5\text{ g} \times 2$)
Mass of additional protein needed: $38\text{ g}$ ($45\text{ g} – 7\text{ g}$)
Explanation: Since the table provides data for $100\text{ g}$, we double the protein for $200\text{ g}$. Subtracting what was already eaten from the goal of $45\text{ g}$ gives the remaining requirement.

6(b)(ii) Importance of fats: Source/store of energy; insulation; protection of vital organs.
Explanation: Fats provide a concentrated energy source ($37\text{ kJ/g}$). They also form a layer of adipose tissue that keeps us warm and cushions internal organs from physical damage.

6(b)(iii) Source of fibre: Whole grain products, fruit, vegetables, beans, or nuts.
Explanation: Fibre (cellulose) comes from plant cell walls, which is why it is found in plant-based foods rather than animal products like yoghurt.

6(c)(i) Equation: $\text{glucose} \rightarrow \text{alcohol (ethanol)} + \text{carbon dioxide}$
Explanation: In the absence of oxygen, yeast breaks down sugar into ethanol and $CO_2$. This is also known as fermentation.

6(c)(ii) Role in bread: Carbon dioxide gas is produced which gets trapped in the dough, making it rise.
Explanation: The $CO_2$ bubbles expand during baking, creating the light, airy texture of bread. The alcohol produced usually evaporates during the baking process.

6(c)(iii) Other use: Production of biofuel (ethanol).
Explanation: Yeast is used on an industrial scale to ferment plant sugars into ethanol, which is used as a renewable alternative to fossil fuels.

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