Home / iGCSE Biology (0610)-5.1 Enzymes – iGCSE Style Questions Paper 2

iGCSE Biology (0610)-5.1 Enzymes – iGCSE Style Questions Paper 2

Question

The diagram shows a process involving an enzyme in the human stomach.

The diagram shows another process, $R$, that affects this enzyme.

Which row identifies the substrate and the cause of process $R$?

▶️ Answer/Explanation
In the first diagram, the molecule labeled $1$ is the substrate because it binds to the enzyme’s active site and is broken down into products labeled $2$. Since the enzyme is located in the human stomach, it is likely pepsin, which functions optimally in acidic conditions ($pH$ $2$). Process $R$ shows the active site changing shape, a process known as denaturation. This happens when the enzyme is exposed to unfavorable conditions, such as alkaline conditions, which would disrupt the enzyme’s structure and render it inactive.
Answer: (B)

Question

What is the correct definition of enzymes?

(A) carbohydrates that act as biological catalysts
(B) carbohydrates that act as substrates
(C) proteins that act as biological catalysts
(D) proteins that act as substrates
▶️ Answer/Explanation

Enzymes are specialized proteins synthesized by living cells to speed up metabolic reactions. They function as biological catalysts, meaning they lower the activation energy required for a reaction to occur without being consumed in the process. While carbohydrates serve as energy sources or structural components, they do not possess catalytic properties. Similarly, a substrate is the specific molecule that an enzyme acts upon, rather than the enzyme itself. Therefore, the most accurate definition identifies them as protein-based catalysts.

Answer: (C)

Question

The graph shows how enzyme activity is affected by temperature.

Why is enzyme activity lower at $55$ °C than it is at $40$ °C?

(A) Heat has killed the enzyme.
(B) The enzyme has been used up.
(C) The reactants are moving faster.
(D) The substrate is less likely to fit into the active site.
▶️ Answer/Explanation
Enzymes are proteins with specific 3D shapes, including an active site perfectly molded for a substrate. As temperature rises beyond the optimum (approx. $40$ °C in this graph), the increased kinetic energy causes the chemical bonds holding the protein together to vibrate and eventually break. This process, called denaturation, alters the shape of the active site. Because the substrate can no longer fit into the changed active site, the rate of reaction drops sharply. Note that enzymes are biological molecules, not living organisms, so they are “denatured” rather than “killed.”
Answer: (D)
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