CIE iGCSE Biology Paper 2 Prediction - 2025
CIE iGCSE Biology Paper 2 Prediction – 2025
Preparing for your IGCSE exam can be daunting, but with the right approach, you can achieve your goals with CIE iGCSE Biology Paper 2 Prediction
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iGCSE Practice Questions, Past Papers , Flashcards and notes available for iGCSE Students at IITian Academy.
Question 1
What is a description of excretion?
A) a chemical reaction that releases energy
B) a permanent increase in size and dry mass
C) removal of waste products of metabolism
D) taking in materials for energy, growth and development
▶️Answer/Explanation
Ans: C
Question 2
The diagram shows how Homo sapiens (modern people) could have evolved from earlier ancestors. Which statement about modern people and their ancestors is correct?
A They are the same species and the same genus.
B They are the same species but not the same genus.
C They are the same genus but not the same species.
D They are neither the same species nor the same genus.
▶️Answer/Explanation
Ans C
Question 3
The diagram shows an animal.
Using the key, which genus does the animal belong to?
▶️Answer/Explanation
Ans: C
Question 4
Which group of living organisms includes the myriapods?
A arthropods
B prokaryotes
C protoctists
D vertebrates
▶️Answer/Explanation
Ans: A
Question 5
The diagram shows a liver cell.
Which features are present in this cell and also in most plant cells?
A cell membrane and cytoplasm
B cell membrane and sap vacuole
C cell wall and cytoplasm
D cell wall and sap vacuole
▶️Answer/Explanation
Ans: A
Question 6
The diagram shows an epidermal cell from an onion plant. The distance between X and Y on the diagram is 60 mm. The actual length of the cell between X and Y is 150 μm.
What is the magnification of the cell?
A ×40
B ×250
C ×400
D ×2500
▶️Answer/Explanation
Ans C
Question 7
The graph shows how the turgor pressure inside a plant cell varies when the cell is placed in external solutions of different concentrations.
What is the state of the cell at point X?
(A) It has gained water and is flaccid.
(B) It has gained water and is turgid.
(C) It has lost water and is flaccid.
(D) It has lost water and is turgid.
▶️Answer/Explanation
Ans: C
Question 8
A test was performed on a food substance. A positive result was shown by a colour change to blue-black.
What solution was used for the test?
A) biuret solution
B) DCPIP solution
C) iodine solution
D) Benedict’s solution
▶️Answer/Explanation
Ans: C
Question 9
Which statement about enzymes is correct?
(A) They are made of carbohydrates.
(B) Their activity is unaffected by pH.
(C) They are used up during the reaction.
(D) They have a complementary shape to their substrate.
▶️Answer/Explanation
Ans: D
Question 10
Two test-tubes were filled with hydrogencarbonate indicator. An aquatic plant was placed into each test-tube and the test-tubes were sealed with bungs, as shown.
Test-tube X was illuminated and test-tube Y was kept in the dark. The results are shown.
What causes the colour changes in the hydrogencarbonate indicator in X and Y?
▶️Answer/Explanation
Ans: B
Question 11
A doctor tested the urine of four patients for both glucose and protein. The colours of the urine samples after testing are shown in the table.
Which patient’s urine contained both protein and glucose?
▶️Answer/Explanation
Ans: C
Question 12
The diagram shows part of the alimentary canal.
Which row correctly identifies the structures labelled J to M?
▶️Answer/Explanation
Ans: A
Question 13
The small intestine is where most digested foods are absorbed. The small intestine has many villi. These are some features of villi.
1 Each villus contains a network of capillaries.
2 Each villus contains a lacteal.
3 The surface of each epithelial cell is covered with microvilli.
4 Villi increase the internal surface area of the small intestine.
Which statements about the villi are important for the efficient absorption of glucose?
A 1, 2, 3 and 4
B 1, 3 and 4 only
C 1 and 3 only
D 2 and 4 only
▶️Answer/Explanation
Ans B
Question 14
The list contains statements about villi.
1. Villi increase the efficiency of the absorption of nutrients.
2. Villi increase the efficiency of the alveoli during gas exchange.
3. Villi provide a large surface area.
4. Villi filter urea from the blood.
Which two statements explain the role of the villi in humans?
A) 1 and 2
B) 1 and 3
C) 2 and 4
D) 3 and 4
▶️Answer/Explanation
Ans B
Question 15
Which region in the leaf of a green plant contains phloem?
A palisade mesophyll
B spongy mesophyll
C upper epidermis
D vascular bundle
▶️Answer/Explanation
Ans D
Question 16
A student set up the experiment shown in the diagram on a hot and dry day. The air bubble moved towards the leafy shoot by 30 mm in 1 hour.
The student was asked to predict and explain the result that would have been obtained on a hot and humid day.
Which row is correct?
▶️Answer/Explanation
Ans: B
Question 17
Which statement describes the process of translocation?
A) the movement of sucrose and amino acids in the xylem from the source to the sink
B) the movement of sucrose and water in the phloem from the sink to the source
C) the movement of sucrose and amino acids in the phloem from the source to the sink
D) the movement of water and amino acids in the xylem from the source to the sink
▶️Answer/Explanation
Ans: C
Question 18
The diagram shows the external structure of the human heart.
Which part is blocked in coronary heart disease?
▶️Answer/Explanation
Ans: B
Question 19
Which substance has a higher concentration in the renal vein than in the renal artery?
A carbon dioxide
B glucose
C oxygen
D urea
▶️Answer/Explanation
Ans A
Question 20
The diagram shows a component of blood.
What is the name of this component?
A lymphocyte
B phagocyte
C platelet
D red blood cell
▶️Answer/Explanation
Ans: B
Question 21
What is a feature of passive immunity?
A Antibodies are acquired from another individual.
B It can be gained through infection with a pathogen.
C Long-term protection is gained.
D Memory cells are produced.
▶️Answer/Explanation
Ans A
Question
What is the approximate percentage of oxygen in expired air?
(A) 0.04%
(B) 4%
(C) 16%
(D) 21%
▶️Answer/Explanation
Ans C
Question 22
Two molecules of glucose are aerobically respired. How many molecules of water are produced?
A 1
B 2
C 6
D 12
▶️Answer/Explanation
Ans D
Question 23
Which labelled organ has a major role in the assimilation of amino acids by converting them to proteins?
▶️Answer/Explanation
Ans A
Question 24
A growing seedling was pinned sideways onto a wooden board that was covered in wet blotting
paper.
The seedling was kept in a dark box.
The diagram shows the seedling after 24 hours. Auxin had become concentrated on the lower
side of the shoot and the root.
What can be concluded from this experiment about the effect of auxin on the seedling?
A A high concentration of auxin stimulates cell elongation on the lower side of the root.
B A high concentration of auxin stimulates cell elongation on the lower side of the shoot.
C A low concentration of auxin stimulates cell elongation on the upper side of the shoot.
D A low concentration of auxin stimulates cell elongation on the upper sides of the shoot and the root.
▶️Answer/Explanation
Ans: B
Question 25
Which statement about antibiotics is correct?
A) Antibiotics are produced by memory cells.
B) Antibiotics are used to treat rickets.
C) Antibiotics are used to treat viral infections.
D) Antibiotics should only be used when essential.
▶️Answer/Explanation
Ans: D
Question 26
The diagram shows a strawberry plant.
Which labelled part of the plant can only be produced by asexual reproduction?
▶️Answer/Explanation
Ans: D
Question 27
The graph shows the concentration of four hormones during the menstrual cycle.
Which line represents progesterone?
▶️Answer/Explanation
Ans: B
Question 28
The table shows the number of new HIV infections in 1990 and 2021 in one country.
Which statement could explain the change in the number of new HIV infections between 1990
and 2021?
A Condoms, which prevent the transmission of STIs, increased in price between 1990 and 2021.
B People in 2021 had more sexual partners.
C Testing of donated blood for pathogens was introduced in 1990.
D There was an increase in drug abuse between 1990 and 2021.
▶️Answer/Explanation
Ans: C
Question 29
The diagram shows the stages of protein synthesis in a cell.
Which row identifies molecules 1, 2 and 3?
▶️Answer/Explanation
Ans: C
Question 30
The diagram shows a food chain.
What is the correct pyramid of numbers for this food chain?
▶️Answer/Explanation
Ans: A
Question 31
The diagram shows the unspecialised cells of a mammalian embryo soon after fertilisation.
What is the correct description of these cells?
(A) embryo cells undergoing meiosis
(B) gametes undergoing mitosis
(C) stem cells undergoing mitosis
(D) zygote undergoing meiosis
▶️Answer/Explanation
Ans: A
Question 32
The graph shows how the mass of DNA per cell changes before, during and after a nuclear division in a diploid cell.
Which row describes the type of nuclear division and the chromosome number of the daughter cells shown in the graph? 
▶️Answer/Explanation
Ans C
Question 33
What is the main source of energy input to biological systems?
A) glucose
B) oxygen
C) plants
D) the Sun
▶️Answer/Explanation
Ans: D
Question 34
Which diagram correctly shows the flow of energy?
▶️Answer/Explanation
Ans: A
Question 35
The diagram shows the nitrogen cycle in the ocean.
Which stage represents nitrification?
▶️Answer/Explanation
Ans: A
Question 36
What is defined as ‘all of the populations of different species in an ecosystem’?
A community
B environment
C habitat
D trophic level
Answer/Explanation
Ans: A
Question 37
What are two reasons for organisms becoming endangered?
- habitat destruction
- monitoring
- education
- pollution
- capitive breeding programmes
A) 1 and 4
B) 1 and 5
C) 2 and 3
D) 3 and 4
▶️Answer/Explanation
Ans: A
Question 38
How will these natural processes be affected by deforestation?
▶️Answer/Explanation
Ans: D
Question 39
The diagram shows the sticky end of theDNA of a plasmid that has been cut by a restriction enzyme.
The unpaired bases are labelled.
What is the correct sequence of the missing bases?
A) CAGTG
B) GTGAC
C) GTCAC
D) CACTG
▶️Answer/Explanation
Ans: C
Question 40
The diagram shows the structure of human skin.
What are X, Y and Z?
▶️Answer/Explanation
Ans: C