CIE iGCSE Co-Ordinated Science B5 Enzymes Exam Style Questions Paper 1
Question
Some species of bacteria live in alkaline hot springs at temperatures of 80 °C.
Which conditions will best suit the enzymes of these bacteria?

▶️ Answer/Explanation
✅ Answer: (D)
Question
Which type of molecules are enzymes?
A. carbohydrates
B. fats
C. hormones
D. proteins
▶️ Answer/Explanation
Carbohydrates and fats are not enzymes, and hormones are chemical messengers, not catalysts.
✅ Answer: (D)
Question
Which statements about enzymes are correct?
- Enzymes are biological catalysts.
- Enzymes are made of fat.
- Enzymes are made of protein.
A. 1 and 2
B. 1 and 3
C. 1 only
D. 3 only
▶️ Answer/Explanation
✅ Answer: (B)
Question
The graphs show the possible effects of temperature on enzyme activity. Which graph is correct for a human enzyme?

▶️ Answer/Explanation
Human enzymes have an optimum temperature of around \(37^\circ\text{C}\) (body temperature). As temperature increases from \(0^\circ\text{C}\) to the optimum, enzyme activity increases because particles have more kinetic energy, leading to more frequent successful collisions between enzyme and substrate. Above the optimum temperature, the enzyme’s active site changes shape (denaturation) and enzyme activity decreases rapidly. Graph A correctly shows this bell-shaped pattern — activity rises to a peak at the optimum temperature and then falls.
Graph B shows decreasing activity from the start (incorrect), Graph C shows a plateau after the optimum (incorrect), and Graph D shows continuously increasing activity (incorrect).
✅ Answer: (A)
Question
Which statements are correct for all enzymes?
- They are proteins.
- They are not affected by temperature.
- They increase the rate of chemical reactions.
- They work best at a high pH.
A. 1, 2 and 4
B. 1, 3 and 4
C. 1 and 3 only
D. 2 and 4 only
▶️ Answer/Explanation
They are affected by temperature (not 2) and each enzyme has an optimum pH, which is not always high (not 4). Thus only 1 and 3 are correct.
✅ Answer: (C)
