iGCSE Biology (0610)-21.3 Genetic modification – iGCSE Style Questions Paper 2

Question

The diagram shows five stages in genetic engineering.

           

        Which stages involve the formation of sticky ends?
         A 1, 2 and 3              B 1 and 2 only              C 1 and 3 only            D 2 and 3 only

Answer/Explanation

Ans

B

Question

Human insulin can be produced in large quantities by modified E. coli bacteria.
      Four of the steps in this production process are listed.
                  1 Insulin is removed from the bacterial culture.
                  2 An enzyme is used to cut out the insulin gene from a human chromosome.
                  3 The insulin gene is placed into the plasmid of the bacterium.
                  4 Bacteria with the insulin gene reproduce very rapidly.
What is the order of these steps?
    A 1 → 2 → 3 → 4
    B 1 → 3 → 4 → 2
    C 2 → 3 → 4 → 1
    D 4 → 1 → 2 → 3

Answer/Explanation

Ans

C

Question

Which statement describes the role of DNA ligase in genetic engineering?
        cuts open a bacterial plasmid
        B  joins the human gene to the plasmid
        inserts plasmid back into bacterium
        D  isolates a human gene

Answer/Explanation

Ans: B

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