Question
The diagram shows five stages in genetic engineering.
Which stages involve the formation of sticky ends?
A 1, 2 and 3 B 1 and 2 only C 1 and 3 only D 2 and 3 only
Answer/Explanation
Ans
B
Question
Human insulin can be produced in large quantities by modified E. coli bacteria.
Four of the steps in this production process are listed.
1 Insulin is removed from the bacterial culture.
2 An enzyme is used to cut out the insulin gene from a human chromosome.
3 The insulin gene is placed into the plasmid of the bacterium.
4 Bacteria with the insulin gene reproduce very rapidly.
What is the order of these steps?
A 1 → 2 → 3 → 4
B 1 → 3 → 4 → 2
C 2 → 3 → 4 → 1
D 4 → 1 → 2 → 3
Answer/Explanation
Ans
C
Question
Which statement describes the role of DNA ligase in genetic engineering?
A cuts open a bacterial plasmid
B joins the human gene to the plasmid
C inserts plasmid back into bacterium
D isolates a human gene
Answer/Explanation
Ans: B